First off, "or a 50 or 100 watt band installed on the radio", I have never heard of this and have no clue what you are talking about. Lets pretend we never even talked of this. I would also wait on an amplifier since you should know what you are doing before you decide to install an illegal product like a cb amplifier.
The most important part of your set-up is the antenna system. Are you going to run one antenna or go co-phased with two? The Wilson is a great antenna choice. Make sure you have good aftermarket coax, or a co-phased harness if you plan on running two antennas. The new factory coax is not really what you want to use anymore.
The antenna needs to have the SWR set properly. Your shooting for anything from 1.0-1.5 . You might have to add some ground strap to your mirrior mount down to the frame of the truck.
Next up is have a good tech peak, tune, and align your radio. You don't want anything clipped or removed. Talk to the tech BEFORE he opens up your radio and find out what he plans on doing and how he does it. You might also consider a better mic, I hate stock mic's.